LC-MS for peptide separation and characterization

  • NMR

    Bruker Avance 600 and 700 magnet

  • Tecan and plate reader

    Tecan robot and Infinite F500 plate reader

  • Small Molecules

    Experimental and computational approaches to drug design

  • NMR Sample Changer

    Temperature controlled, 96-tubes sample racks can be handled in automation

Structural Biology

Research in our laboratory aims to establish the structural basis of the mechanism of action of different peptide hormones.

Biophysical Chemistry

Studies of the interaction of the hormones with their G-protein coupled receptors employ a combination of spectroscopic techniques (NMR, EPR, fluorescence, CD), photoaffinity labeling, and extensive computer simulations.

Rational Drug Design

The structures provided from these efforts have facilitated the rational design of molecules with enhanced potency, receptor specificity, and duration of action.

NMR Spectroscopy

High-resolution NMR methods provide structural features of the protein domains, both while free and bound to the target receptor.

PLoSOne 2015: Interaction of cFLIP and Calmodulin

PLoS One. 2015 Nov 3;10(11):e0141692. doi: 10.1371/journal.pone.0141692. eCollection 2015. Identification and Characterization...

04th Jan

SCIENTIFIC REPORTS 2015: “A novel caspase 8 selective small molecule potentiates TRAIL-induced cell death”

Scientific Reports 5, Article number: 9893 doi:10.1038/srep09893 A novel caspase 8 selective...

11th May

J Biol Chem. 2015: “Phosphorylation of Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) by Akt Promotes Stability and Mitogenic Function of S-phase Kinase-associated Protein-2 (Skp2).”

J Biol Chem. 2015 290(5):2879-87.  doi: 10.1074/jbc.M114.609768. PMID: 25492869 “Phosphorylation of Ezrin-Radixin-Moesin-binding Phosphoprotein...

24th Feb

J Pept Sci. 2014: “Small-molecule inhibitors of JC polyomavirus infection.”

J Pept Sci. 2014 Dec 19. doi: 10.1002/psc.2731. [Epub ahead of print]...

24th Feb

Biochemistry. 2014: “Protein Engineering of the N-terminus of NEMO: structure stabilization and rescue of IKKβ binding”

Biochemistry. 2014  Nov 4;53(43):6776-85. doi: 10.1021/bi500861x. Epub 2014 Oct 23 Protein Engineering of the...

23rd Oct

Virus Rs. 2014: “Gallic acid-based small-molecule inhibitors of JC and BK polyomaviral infection.”

Virus Res. 2014 Aug 30;189:280-5. doi: 10.1016/j.virusres.2014.06.008. Epub 2014 Jun 21. Gallic...

24th Sep
Virus Rs. 2014: “Gallic acid-based small-molecule inhibitors of JC and BK polyomaviral infection.”

Biochemistry 2014 doi: 10.1021/bi500368k

Biochemistry. 2014 Sep 23;53(37):5916-22. doi: 10.1021/bi500368k. Epub 2014 Sep 15. Small Molecule Inhibition...

19th Sep

Chemical Communications 2014 doi: 10.1039/c4cc04399e.

Chem Commun (Camb). 2014 Sep 16;50(81):12037-9. doi: 10.1039/c4cc04399e. Monitoring ATP hydrolysis and...

19th Sep

Biochemistry 2012 doi: 10.1021/bi300639d.

Reining in polyoma virus associated nephropathy: design and characterization of a template...

14th Mar

Proteins publication

Engineering a soluble parathyroid hormone GPCR mimetic. Proteins. 2013 Dec 27. doi:...

14th Mar