Moss Methods

Our Moss Manual

The moss Physcomitrella patens is a powerful model system to study plant gene function. Unique among plants, moss performs efficient homologous recombination enabling precise genetic manipulation, including gene deletions and allele replacements. With a completely sequenced genome, facile genetic alteration, and a relatively simple developmental pattern, moss provides an unparalleled tool for dissecting plant gene function. Moss is simple to propagate and transform, and requires little space to maintain. This lab manual contains information about propagation, transformation, and DNA and protein isolation.  Moss Manual
(Updated: 2012)

Moss Propagation and Culture

Moss should be propagated weekly. A detailed explanation can be found in the moss manual. Watch the video for a brief summary on moss propagation. Propagation Video

Protoplasting

By removing the cell walls from moss tissue, it is possible to generate a suspension of single cells called protoplasts. Moss protoplasts can be transformed with DNA and then regenerated into whole plants. For details on this procedure, please refer to the moss manual.

Common Assays

Available Reagents: Plasmids