Overexpression Vectors

Transient and/or Stable Overexpression Vectors

Create expression of your favorite protein using the maize ubiquitin promoter. Can be used for transient overexpression or for generating a stable line. All vectors have moss sequences for homologous recombination-based targeting. When exogenous sequences are inserted into these loci, there is no affect on growth or development. Cloning into these vectors is mediated by Gateway™ recombination-based cloning from Invitrogen.

Download Sequences

pTH-UBI-Gate

Hygromycin resistant in plants, targets the Pp108 locus.

pTK-UBI-Gate

Kanamycin resistant in plants, targets a redundant copy of the ARPC2 gene.

pTZ-UBI-Gate

Zeocin resistant in plants, targets a redundant copy of the ARPC3 gene.

These plasmids are available upon request. Each plasmid has a graphic map, raw sequence, and an annotated SerialCloner (.xdna) file available for download. SerialCloner is free to download here. General disclaimer: plasmid sequences are not always perfect. When we know of a discrepancy, we will try and note it.