Two-Fragment Recombination Vectors

Two-Fragment Recombination Vectors

For generating epitope-tagged versions of your favorite protein. For details, refer to our moss manual.

The destination vectors for these entry vectors include any Gateway™-enabled destination vector (i.e. pTH-UBI-gate, pTK-UBI-gate, pZeo-UBI-gate). Click here to view these vectors.

Download Sequences

mEGFP-L1R5

Element 1 for N-terminal tagging.

mEGFP-L5L2

Element 2 for C-terminal tagging.

mCherry-L1R5

Element 1 for N-terminal tagging.

mCherry-L5L2

Element 2 for C-terminal tagging.

3xFLAG-L5L2

Element 2 for C-terminal tagging.

These plasmids are available upon request. Each plasmid has a graphic map, raw sequence, and an annotated SerialCloner (.xdna) file available for download. SerialCloner is free to download here. General disclaimer: plasmid sequences are not always perfect. When we know of a discrepancy, we will try and note it.