Two-Fragment Recombination Vectors
For generating epitope-tagged versions of your favorite protein. For details, refer to our moss manual.
The destination vectors for these entry vectors include any Gateway™-enabled destination vector (i.e. pTH-UBI-gate, pTK-UBI-gate, pZeo-UBI-gate). Click here to view these vectors.
mEGFP-L1R5Element 1 for N-terminal tagging. |
mEGFP-L5L2Element 2 for C-terminal tagging. |
mCherry-L1R5Element 1 for N-terminal tagging. |
mCherry-L5L2Element 2 for C-terminal tagging. |
3xFLAG-L5L2Element 2 for C-terminal tagging. |
These plasmids are available upon request. Each plasmid has a graphic map, raw sequence, and an annotated SerialCloner (.xdna) file available for download. SerialCloner is free to download here. General disclaimer: plasmid sequences are not always perfect. When we know of a discrepancy, we will try and note it.