Molecular Interactions & Imaging Core (MIIC)

Location:
Vail Building, Room 309
Geisel School of Medicine, Hanover, NH
603.650.1605

INTERACTIONS AND IMAGING

The Molecular Interactions and Imaging Core (MIIC) enables research projects by offering support on numerous methodologies to characterize biomolecular interactions. In particular, we provide expertise on sample preparation (working with the MTC), data collection, and analysis for a range of instrumentation including surface plasmon resonance (label-free analysis of biomolecular interactions) and numerous measurements using absorbance, luminescence, and fluorescence (fluorescence anisotropy, alpha-screen, near-infrared fluorescence). The MIIC can facilitate access to additional instrumentation and various applications to characterize biological systems, including drug target identification and validation, such as isothermal calorimetry and nuclear magnetic resonance.

 

AVAILABLE EQUIPMENT IN THE MIIC:

(for more information, including additional equipment details, reserving equipment, and  requesting training, visit RaDar)

EquipmentDescription
Biacore X100 Measurement of biomolecular interactions in real-time.
LI-COR Odyssey ClxFor quantitative, highly sensitive near-infrared fluorescence detection.
Bio-Rad GelDocXRFor high-resolution nucleic acid and protein gel imaging.
Synergy Neo2 Hybrid Multi-Mode Microplate Reader For numerous HTS applications with multi-detection modes.
DeNovix DS-11 FX+Spectrophotometer/FluorometerFor a variety of micro-volume nucleic acid and protein analyses.

MICROSCOPY

Contact: Zdenek Svindrych, Ph.D.

We provide microscopy support to the bioMT community.  With a broad range of microscopes available at Dartmouth (either located in one of the imaging cores – Class of 1978 Life Sciences Center and Dartmouth-Hitchcock Medical Center, or in individual labs), we can cover many applications. The MIIC uses microscopy for both biochemical and cellular studies and assists investigators with designing and implementing methodologies to answer specific research questions.

AVAILABLE MICROSCOPES:

MicroscopesDescription
Laser scanning confocal Nikon A1RSi with FLIM/FCS, Zeiss LSM880 with Airy-scan, Zeiss LSM800 with Airyscan
Spinning disk confocal Andor Dragonfly 302, Andor W1 with Mosaic and Micropointphotostimulation/photoablation
Total Internal ReflectionNikon/Andor TIRF, Olympus TIRF
MutliphotonOlympus FVMPE-RS with MaiTai InSight FLIM/FCS
EpifluorescenceNikon Ti-E - inverted, Nikon 90i – upright, Nikon SMZ1500 – dissecting stereo zoom
AVAILABLE IMAGE-PROCESSING SOFTWARE
Imaris workstationWith Measurement Pro, ImarisTrack, ImarisColoc, ImarisXT, ImarisCell, ImarisVantage, and Filament Tracker
NIS Elements workstationWith Deconvolution