Common Assays
Growth AssayMoss protoplasts given the proper growth conditions will regenerate into a whole plant within a few days. Thousands of plants can be regenerated. Taking pictures of the plants, it is possible to get measurements of overall plant size and shape.
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RNAi AssayMoss performs RNAi-induced gene silencing. Double stranded RNA can be generated by transformation of DNA constructs containing inverted repeats of the sequence targeted for gene silencing. Our laboratory uses a robust RNAi assay that enables rapid identification of gene function.
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Complementation AssayComplementation studies provide validation of loss-of-function studies and mechanistic insights into gene function. By targeting untranslated regions of sequence, RNAi-induced phenotypes can be rescued by expression of a construct containing only the open reading frame.
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