Sample of Lab Protocols

Here are some of the protocols and techniques our lab has used over the years. Please feel free to contact one of our lab members if interested in the current research and techniques we use!

Plasmid Miniprep from S. aureus

Plasmid Miniprep from E. coli

pMAD Allelic Exchange Protocol

RNA extraction for Northern Blots

Northern Blot Probe Synthesis

Northern Blot Protocol

Minimum Inhibitory Concentration (MIC) Assay

S. aureus cDNA Labeling

Transient Transfection of COS7 cells to analyze by Immuno-Fluorescense (from Stanton Lab)

Intracellular Vesicle Staining

Protocol for Hybridomas

Genomic DNA Preparation for S. aureus

Gel Extraction Protocol

Fast Colony DNA extraction Protocol

Development of Western Blot

Western Blot Protocol (ECL)

Big Dye Sequencing

Bacterial Invasion Protocol

Transformation of S. aureus cells

Southern Blot Protocol

Southern Blot with DIG Probes

Southwestern Blot Protocol

Protocol for thawing cells stocks from Nitrogen Bath