Phys Med Biol. 2012 Oct 21;57(20):6647-59. doi: 10.1088/0031-9155/57/20/6647. Epub 2012 Oct 1. ABSTRACT The quantification of tumor molecular expression in vivo could have a significant impact for informing and monitoring emerging targeted therapies in oncology. Molecular imaging of targeted tracers can be used to quantify receptor expression in the form of a binding potential (BP)…Continue Reading Advantages of a dual-tracer model over reference tissue models for binding potential measurement in tumors
Computed tomography-guided time-domain diffuse fluorescence tomography in small animals for localization of cancer biomarkers
J Vis Exp. 2012 Jul 17;(65):e4050. doi: 10.3791/4050. ABSTRACT Small animal fluorescence molecular imaging (FMI) can be a powerful tool for preclinical drug discovery and development studies. However, light absorption by tissue chromophores (e.g., hemoglobin, water, lipids, melanin) typically limits optical signal propagation through thicknesses larger than a few millimeters. Compared to other visible wavelengths,…Continue Reading Computed tomography-guided time-domain diffuse fluorescence tomography in small animals for localization of cancer biomarkers
Nanoparticle uptake in tumors is mediated by the interplay of vascular and collagen density with interstitial pressure
Nanomedicine. 2013 Feb;9(2):151-8. doi: 10.1016/j.nano.2012.07.002. Epub 2012 Jul 25. ABSTRACT Nanoparticle delivery into solid tumors is affected by vessel density, interstitial fluid pressure (IFP) and collagen, as shown in this article by contrasting the in vivo macroscopic quantitative uptake of 40 nm fluorescent beads in three tumor types.The fluorescence uptake was quantified on individual animals…Continue Reading Nanoparticle uptake in tumors is mediated by the interplay of vascular and collagen density with interstitial pressure
Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging
J Biomed Opt. 2012 Jun;17(6):066001. doi: 10.1117/1.JBO.17.6.066001. ABSTRACT In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three…Continue Reading Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging