Examining the Feasibility of Quantifying Receptor Availability Using Cross-Modality Paired-Agent Imaging
Boyu Meng, Negar Sadeghipour, Margaret R. Folaron, Rendall R. Strawbridge, Kimberley S. Samkoe, Kenneth M. Tichauer & Scott C. Davis Mol Imaging Biol. 2021 Jul 20. doi: 10.1007/s11307-021-01629-6. Online ahead of print. ABSTRACT PURPOSE: The ability to noninvasively quantify receptor availability (RA) in solid tumors is an aspirational goal of molecular imaging, often challenged by the…Continue Reading Examining the Feasibility of Quantifying Receptor Availability Using Cross-Modality Paired-Agent Imaging
A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models
Elif Kayaalp-Nalbant, Cody Rounds, Negar Sadeghipour, Boyu Meng, Margaret R. Folaron, Chandrika Haldar, Rendall R. Strawbridge, Kimberley S. Samkoe, Scott C. Davis, Kenneth M. Tichauer Proc SPIE Int Soc Opt Eng. 2020 Feb;11219:112190G. doi: 10.1117/12.2545182. Epub 2020 Feb 19. ABSTRACT A paired-agent fluorescent molecular imaging strategy is presented as a method to measure drug target engagement in whole tumor imaging. The protocol involves dynamic…Continue Reading A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models
Effect of nonspecific binding of imaging agents to plasma protein in the paired-agent imaging for resection during surgery (PAIRS)
Xiaochun Xu 1, Kenneth M Tichauer 2, Kimberley S Samkoe 3 Proc SPIE Int Soc Opt Eng. 2020 Feb;11219:112190P. Epub 2020 Feb 19. ABSTRACT Long-term survival of head and neck squamous cell carcinoma (HNSCC) patients have proven to be correlated with negative surgical margins. Paired-Agent Imaging for Resection during Surgery (PAIRS) is capable of drawing the fine line between tumor…Continue Reading Effect of nonspecific binding of imaging agents to plasma protein in the paired-agent imaging for resection during surgery (PAIRS)
TRIPODD: a Novel Fluorescence Imaging Platform for In Situ Quantification of Drug Distribution and Therapeutic Response
Nathan P. McMahon, Allison Solanki, Lei G. Wang, Antonio R. Montaño, Jocelyn A. Jones, Kimberley S. Samkoe, Kenneth M. Tichauer & Summer L. Gibbs Mol Imaging Biol. 2021 Mar 9. doi: 10.1007/s11307-021-01589-x. Online ahead of print. ABSTRACT PURPOSE: Personalized medicine has largely failed to produce curative therapies in advanced cancer patients. Evaluation of in situ drug target availability (DTA) concomitant with local…Continue Reading TRIPODD: a Novel Fluorescence Imaging Platform for In Situ Quantification of Drug Distribution and Therapeutic Response
Intraoperative Detection of Micrometastases in Whole Excised Lymph Nodes Using Fluorescent Paired-Agent Imaging Principles: Identification of a Suitable Staining and Rinsing Protocol
Chengyue Li, Veronica C. Torres, Yusheng He, Xiaochun Xu, Yusairah Basheer, Georgia Papavasiliou, Kimberley S. Samkoe, Jovan G. Brankov & Kenneth M. Tichauer Mol Imaging Biol. 2021 Aug;23(4):537-549. doi: 10.1007/s11307-021-01587-z. Epub 2021 Feb 16. ABSTRACT PURPOSE: Correctly identifying nodal status is recognized as a critical prognostic factor in many cancer types and is essential to guide adjuvant treatment. Currently, surgical removal of lymph nodes…Continue Reading Intraoperative Detection of Micrometastases in Whole Excised Lymph Nodes Using Fluorescent Paired-Agent Imaging Principles: Identification of a Suitable Staining and Rinsing Protocol
Task-based evaluation of fluorescent-guided cancer surgery as a means of identifying optimal imaging agent properties in the context of variability in tumor- and healthy-tissue physiology
Kenneth M. Tichauer, Cheng Wang, Xiaochun Xu, Kimberley S. Samkoe Proc SPIE Int Soc Opt Eng. 2020 Feb;11222:112220O. doi: 10.1117/12.2546700. Epub 2020 Feb 19. ABSTRACT Fluorescent molecular-guided surgery (FGS) is at a tipping point in terms of clinical approval and adoption in a number cancer applications, with ongoing phase 0 and phase 1 clinical trials being carried out…Continue Reading Task-based evaluation of fluorescent-guided cancer surgery as a means of identifying optimal imaging agent properties in the context of variability in tumor- and healthy-tissue physiology
Hyperspectral imaging and spectral unmixing for improving whole-body fluorescence cryo-imaging
Dennis Wirth, Brook Byrd, Boyu Meng, Rendall R. Strawbridge, Kimberley S. Samkoe, and Scott C. Davis Biomed Opt Express. 2020 Dec 16;12(1):395-408. doi: 10.1364/BOE.410810. eCollection 2021 Jan 1. ABSTRACT Whole-animal fluorescence cryo-imaging is an established technique that enables visualization of the biodistribution of labeled drugs, contrast agents, functional reporters and cells in detail. However, many…Continue Reading Hyperspectral imaging and spectral unmixing for improving whole-body fluorescence cryo-imaging
Visualization and quantification of pancreatic tumor stroma in fresh tissue via ultraviolet surface excitation
Phuong Vincent, Petr Bruza, Scott M. Palisoul, Jason R. Gunn, Kimberley S. Samkoe, P. Jack Hoopes, Tayyaba Hasan, Brian W. Pogue J Biomed Opt. 2021 Jan;26(1):016002. doi: 10.1117/1.JBO.26.1.016002. ABSTRACT SIGNIFICANCE: The study has confirmed the feasibility of using ultraviolet (UV) excitation to visualize and quantify desmoplasia in fresh tumor tissue of pancreatic adenocarcinoma (PDAC) in an orthotopic xenograft mouse model, which provides…Continue Reading Visualization and quantification of pancreatic tumor stroma in fresh tissue via ultraviolet surface excitation
Noninvasive quantification of target availability during therapy using paired-agent fluorescence tomography
Boyu Meng1, Margaret R. Folaron1, Rendall R. Strawbridge1, Negar Sadeghipour2, Kimberley S. Samkoe3, Kenneth Tichauer2, Scott C. Davis1 Theranostics. 2020 Sep 14;10(24):11230-11243. doi: 10.7150/thno.45273. eCollection 2020. ABSTRACT Immuno-oncological treatment strategies that target abnormal receptor profiles of tumors are an increasingly important feature of cancer therapy. Yet, assessing receptor availability (RA) and drug-target engagement, important determinants…Continue Reading Noninvasive quantification of target availability during therapy using paired-agent fluorescence tomography