Mol Imaging Biol. 2017 Aug;19(4):512-521. doi: 10.1007/s11307-016-1033-y. ABSTRACT PURPOSE: ABY-029, a synthetic Affibody peptide, Z03115-Cys, labeled with a near-infrared fluorophore, IRDye® 800CW, targeting epidermal growth factor receptor (EGFR) has been produced under good manufacturing practices for a US Food and Drug Administration-approved first-in-use human study during surgical resection of glioma, as well as other tumors….Continue Reading Toxicity and Pharmacokinetic Profile for Single-Dose Injection of ABY-029: a Fluorescent Anti-EGFR Synthetic Affibody Molecule for Human Use
Simultaneous <em>In Vivo</em> Fluorescent Markers for Perfusion, Protoporphyrin Metabolism, and EGFR Expression for Optically Guided Identification of Orthotopic Glioma
Clin Cancer Res. 2017 May 1;23(9):2203-2212. doi: 10.1158/1078-0432.CCR-16-1400. Epub 2016 Oct 31. ABSTRACT Purpose: While extent of tumor resection is an important predictor of outcome in glioma, margin delineation remains challenging due to lack of inherent contrast between tumor and normal parenchyma. Fluorescence-guided surgery is promising for its ability to enhance contrast through exogenous fluorophores;…Continue Reading Simultaneous <em>In Vivo</em> Fluorescent Markers for Perfusion, Protoporphyrin Metabolism, and EGFR Expression for Optically Guided Identification of Orthotopic Glioma
Optical tracer size differences allow quantitation of active pumping rate versus Stokes-Einstein diffusion in lymphatic transport
J Biomed Opt. 2016 Oct 1;21(10):100501. doi: 10.1117/1.JBO.21.10.100501. ABSTRACT Lymphatic uptake of interstitially administered agents occurs by passive convective–diffusive inflow driven by interstitial concentration and pressure, while the downstream lymphatic transport is facilitated by active propulsive contractions of lymphatic vessel walls. Near-infrared fluorescence imaging in mice was used to measure these central components of lymphatic…Continue Reading Optical tracer size differences allow quantitation of active pumping rate versus Stokes-Einstein diffusion in lymphatic transport
Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer
Br J Cancer. 2016 Sep 27;115(7):805-13. doi: 10.1038/bjc.2016.267. Epub 2016 Aug 30. ABSTRACT BACKGROUND: Aminolevulinic acid (ALA)-based photodynamic therapy (PDT) provides selective uptake and conversion of ALA into protoporphyrin IX (PpIX) in actinic keratosis and squamous cell carcinoma, yet large response variations in effect are common between individuals. The aim of this study was to…Continue Reading Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer
Review of fluorescence guided surgery systems: identification of key performance capabilities beyond indocyanine green imaging
J Biomed Opt. 2016 Aug 1;21(8):80901. doi: 10.1117/1.JBO.21.8.080901. ABSTRACT There is growing interest in using fluorescence imaging instruments to guide surgery, and the leading options for open-field imaging are reviewed here. While the clinical fluorescence-guided surgery (FGS) field has been focused predominantly on indocyanine green (ICG) imaging, there is accelerated development of more specific molecular…Continue Reading Review of fluorescence guided surgery systems: identification of key performance capabilities beyond indocyanine green imaging
Fluorescent Affibody Molecule Administered In Vivo at a Microdose Level Labels EGFR Expressing Glioma Tumor Regions
Mol Imaging Biol. 2017 Feb;19(1):41-48. doi: 10.1007/s11307-016-0980-7. ABSTRACT PURPOSE: Fluorescence guidance in surgical oncology provides the potential to realize enhanced molecular tumor contrast with dedicated targeted tracers, potentially with a microdose injection level. For most glioma tumors, the blood brain barrier is compromised allowing some exogenous drug/molecule delivery and accumulation for imaging. The aberrant overexpression…Continue Reading Fluorescent Affibody Molecule Administered In Vivo at a Microdose Level Labels EGFR Expressing Glioma Tumor Regions
Vision 20/20: Molecular-guided surgical oncology based upon tumor metabolism or immunologic phenotype: Technological pathways for point of care imaging and intervention
Med Phys. 2016 Jun;43(6):3143-3156. doi: 10.1118/1.4951732. ABSTRACT Surgical guidance with fluorescence has been demonstrated in individual clinical trials for decades, but the scientific and commercial conditions exist today for a dramatic increase in clinical value. In the past decade, increased use of indocyanine green based visualization of vascular flow, biliary function, and tissue perfusion has…Continue Reading Vision 20/20: Molecular-guided surgical oncology based upon tumor metabolism or immunologic phenotype: Technological pathways for point of care imaging and intervention
Revisiting photodynamic therapy dosimetry: reductionist & surrogate approaches to facilitate clinical success
Phys Med Biol. 2016 Apr 7;61(7):R57-89. doi: 10.1088/0031-9155/61/7/R57. Epub 2016 Mar 10. ABSTRACT Photodynamic therapy (PDT) can be a highly complex treatment, with many parameters influencing treatment efficacy. The extent to which dosimetry is used to monitor and standardize treatment delivery varies widely, ranging from measurement of a single surrogate marker to comprehensive approaches that…Continue Reading Revisiting photodynamic therapy dosimetry: reductionist & surrogate approaches to facilitate clinical success