Category: Publications

J Biomed Opt. 2015 Feb;20(2):26001. doi: 10.1117/1.JBO.20.2.026001. ABSTRACT The ability to image targeted tracer binding to epidermal growth factor receptor (EGFR) was studied in vivo in orthotopically grown glioma tumors of different sizes. The binding potential was quantified using a dual-tracer approach, which employs a fluorescently labeled peptide targeted to EGFR and a reference tracer…Continue Reading Tomography of epidermal growth factor receptor binding to fluorescent Affibody in vivo studied with magnetic resonance guided fluorescence recovery in varying orthotopic glioma sizes

Austin J Biomed Eng. 2014 Mar 5;1(1):austinpublishinggroup.com/biomedical-engineering/fulltext/ajbe-v1-id1002.php. ABSTRACT Molecular differences between cancerous and healthy tissue have become key targets for novel therapeutics specific to tumor receptors. However, cancer cell receptor expression can vary within and amongst different tumors, making strategies that can quantify receptor concentration in vivo critical for the progression of targeted therapies. Recently…Continue Reading Comparison of Kinetic Models for Dual-Tracer Receptor Concentration Imaging in Tumors

Biomed Opt Express. 2014 Aug 29;5(10):3280-91. doi: 10.1364/BOE.5.003280. eCollection 2014 Oct 1. ABSTRACT Ratiometric approaches to quantifying molecular concentrations have been used for decades in microscopy, but have rarely been exploited in vivo until recently. One dual-tracer approach can utilize an untargeted reference tracer to account for non-specific uptake of a receptor-targeted tracer, and ultimately…Continue Reading Pixel-based absorption correction for dual-tracer fluorescence imaging of receptor binding potential

Nat Med. 2014 Nov;20(11):1348-53. doi: 10.1038/nm.3732. Epub 2014 Oct 26. ABSTRACT Lymph node biopsy is employed in many cancer surgeries to identify metastatic disease and to determine cancer stage, yet morbidity and diagnostic delays associated with lymph node biopsy could be avoided if noninvasive imaging of nodal involvement were reliable. Molecular imaging has potential in…Continue Reading Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging

Cancer Res. 2014 Dec 15;74(24):7465-74. doi: 10.1158/0008-5472.CAN-14-0141. Epub 2014 Oct 24. ABSTRACT As receptor-targeted therapeutics become increasingly used in clinical oncology, the ability to quantify protein expression and pharmacokinetics in vivo is imperative to ensure successful individualized treatment plans. Current standards for receptor analysis are performed on extracted tissues. These measurements are static and often…Continue Reading Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach

Proc SPIE Int Soc Opt Eng. 2013 Feb 26;8584:85840F. doi: 10.1117/12.2008067. ABSTRACT Survival of head and neck cancer patients has not improved in several decades despite advances in diagnostic and therapeutic techniques. Tumor hypoxia in head and neck cancers is a critical factor that leads to poor prognosis, resistance to radiation and chemotherapies, and increased…Continue Reading Oxygen microenvironment affects the uptake of nanoparticles in head and neck tumor cells

Phys Med Biol. 2014 May 21;59(10):2341-51. doi: 10.1088/0031-9155/59/10/2341. Epub 2014 Apr 17. ABSTRACT Dual-tracer molecular imaging is a powerful approach to quantify receptor expression in a wide range of tissues by using an untargeted tracer to account for any nonspecific uptake of a molecular-targeted tracer. This approach has previously required the pharmacokinetics of the receptor-targeted…Continue Reading Accounting for pharmacokinetic differences in dual-tracer receptor density imaging

Adv Exp Med Biol. 2014;812:87-95. doi: 10.1007/978-1-4939-0620-8_12. ABSTRACT A hypoxic microenvironment in solid tumors has been known to cause resistance to standard therapies and to increase the malignant potential of tumors. The utilization of magnetic nanoparticle hyperthermia (mNPH) has shown promise in improving therapeutic outcome by (1) killing of hypoxic tumor cells directly and (2)…Continue Reading Modulation of hypoxia by magnetic nanoparticle hyperthermia to augment therapeutic index

Mol Imaging Biol. 2014 Aug;16(4):488-94. doi: 10.1007/s11307-013-0715-y. ABSTRACT PURPOSE: With the goal of facilitating tracer kinetic analysis in small-animal planar fluorescence imaging, an experimental method for characterizing tracer arterial input functions is presented. The proposed method involves exposing the common carotid arteries by surgical dissection, which can then be imaged directly during tracer injection and…Continue Reading Direct characterization of arterial input functions by fluorescence imaging of exposed carotid artery to facilitate kinetic analysis

J Biomed Opt. 2014 Jan;19(1):15005. doi: 10.1117/1.JBO.19.1.015005. ABSTRACT Directional Fourier spatial frequency analysis was used on standard histological sections to identify salient directional bias in the spatial frequencies of stromal and epithelial patterns within tumor tissue. This directional bias is shown to be correlated to the pathway of reduced fluorescent tracer transport. Optical images of…Continue Reading Spatial frequency analysis of anisotropic drug transport in tumor samples