J Biomed Opt. 2016 Oct 1;21(10):100501. doi: 10.1117/1.JBO.21.10.100501. ABSTRACT Lymphatic uptake of interstitially administered agents occurs by passive convective–diffusive inflow driven by interstitial concentration and pressure, while the downstream lymphatic transport is facilitated by active propulsive contractions of lymphatic vessel walls. Near-infrared fluorescence imaging in mice was used to measure these central components of lymphatic…Continue Reading Optical tracer size differences allow quantitation of active pumping rate versus Stokes-Einstein diffusion in lymphatic transport
Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer
Br J Cancer. 2016 Sep 27;115(7):805-13. doi: 10.1038/bjc.2016.267. Epub 2016 Aug 30. ABSTRACT BACKGROUND: Aminolevulinic acid (ALA)-based photodynamic therapy (PDT) provides selective uptake and conversion of ALA into protoporphyrin IX (PpIX) in actinic keratosis and squamous cell carcinoma, yet large response variations in effect are common between individuals. The aim of this study was to…Continue Reading Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer
Review of fluorescence guided surgery systems: identification of key performance capabilities beyond indocyanine green imaging
J Biomed Opt. 2016 Aug 1;21(8):80901. doi: 10.1117/1.JBO.21.8.080901. ABSTRACT There is growing interest in using fluorescence imaging instruments to guide surgery, and the leading options for open-field imaging are reviewed here. While the clinical fluorescence-guided surgery (FGS) field has been focused predominantly on indocyanine green (ICG) imaging, there is accelerated development of more specific molecular…Continue Reading Review of fluorescence guided surgery systems: identification of key performance capabilities beyond indocyanine green imaging
Fluorescent Affibody Molecule Administered In Vivo at a Microdose Level Labels EGFR Expressing Glioma Tumor Regions
Mol Imaging Biol. 2017 Feb;19(1):41-48. doi: 10.1007/s11307-016-0980-7. ABSTRACT PURPOSE: Fluorescence guidance in surgical oncology provides the potential to realize enhanced molecular tumor contrast with dedicated targeted tracers, potentially with a microdose injection level. For most glioma tumors, the blood brain barrier is compromised allowing some exogenous drug/molecule delivery and accumulation for imaging. The aberrant overexpression…Continue Reading Fluorescent Affibody Molecule Administered In Vivo at a Microdose Level Labels EGFR Expressing Glioma Tumor Regions
Vision 20/20: Molecular-guided surgical oncology based upon tumor metabolism or immunologic phenotype: Technological pathways for point of care imaging and intervention
Med Phys. 2016 Jun;43(6):3143-3156. doi: 10.1118/1.4951732. ABSTRACT Surgical guidance with fluorescence has been demonstrated in individual clinical trials for decades, but the scientific and commercial conditions exist today for a dramatic increase in clinical value. In the past decade, increased use of indocyanine green based visualization of vascular flow, biliary function, and tissue perfusion has…Continue Reading Vision 20/20: Molecular-guided surgical oncology based upon tumor metabolism or immunologic phenotype: Technological pathways for point of care imaging and intervention
Revisiting photodynamic therapy dosimetry: reductionist & surrogate approaches to facilitate clinical success
Phys Med Biol. 2016 Apr 7;61(7):R57-89. doi: 10.1088/0031-9155/61/7/R57. Epub 2016 Mar 10. ABSTRACT Photodynamic therapy (PDT) can be a highly complex treatment, with many parameters influencing treatment efficacy. The extent to which dosimetry is used to monitor and standardize treatment delivery varies widely, ranging from measurement of a single surrogate marker to comprehensive approaches that…Continue Reading Revisiting photodynamic therapy dosimetry: reductionist & surrogate approaches to facilitate clinical success
Image-derived arterial input function for quantitative fluorescence imaging of receptor-drug binding in vivo
J Biophotonics. 2016 Mar;9(3):282-95. doi: 10.1002/jbio.201500162. Epub 2015 Sep 9. ABSTRACT Receptor concentration imaging (RCI) with targeted-untargeted optical dye pairs has enabled in vivo immunohistochemistry analysis in preclinical subcutaneous tumors. Successful application of RCI to fluorescence guided resection (FGR), so that quantitative molecular imaging of tumor-specific receptors could be performed in situ, would have a…Continue Reading Image-derived arterial input function for quantitative fluorescence imaging of receptor-drug binding in vivo
Advancing Molecular-Guided Surgery through probe development and testing in a moderate cost evaluation pipeline
Proc SPIE Int Soc Opt Eng. 2015 Mar 4;9311:931112. doi: 10.1117/12.2083224. ABSTRACT Molecular guided oncology surgery has the potential to transform the way decisions about resection are done, and can be critically important in areas such as neurosurgery where the margins of tumor relative to critical normal tissues are not readily apparent from visual or…Continue Reading Advancing Molecular-Guided Surgery through probe development and testing in a moderate cost evaluation pipeline
Nodal lymph flow quantified with afferent vessel input function allows differentiation between normal and cancer-bearing nodes
Biomed Opt Express. 2015 Mar 17;6(4):1304-17. doi: 10.1364/BOE.6.001304. eCollection 2015 Apr 1. ABSTRACT Morbidity and complexity involved in lymph node staging via surgical resection and biopsy could ideally be improved using node assay techniques that are non-invasive. While visible blue dyes are often used to locate the sentinel lymph nodes from draining lymphatic vessels near…Continue Reading Nodal lymph flow quantified with afferent vessel input function allows differentiation between normal and cancer-bearing nodes
Perfusion CT estimates photosensitizer uptake and biodistribution in a rabbit orthotopic pancreatic cancer model: a pilot study
Acad Radiol. 2015 May;22(5):572-9. doi: 10.1016/j.acra.2014.12.014. Epub 2015 Feb 14. ABSTRACT RATIONALE AND OBJECTIVES: It was hypothesized that perfusion computed tomography (CT), blood flow (BF), blood volume (BV), and vascular permeability surface area (PS) product parameters would be predictive of therapeutic anticancer agent uptake in pancreatic cancer, facilitating image-guided interpretation of human treatments. The hypothesis…Continue Reading Perfusion CT estimates photosensitizer uptake and biodistribution in a rabbit orthotopic pancreatic cancer model: a pilot study