Category: Uncategorized

In vivo quantification of tumor receptor binding potential with dual-reporter molecular imaging

Mol Imaging Biol. 2012 Oct;14(5):584-92. doi: 10.1007/s11307-011-0534-y. ABSTRACT PURPOSE: Receptor availability represents a key component of current cancer management. However, no approaches have been adopted to do this clinically, and the current standard of care is invasive tissue biopsy. A dual-reporter methodology capable of quantifying available receptor binding potential of tumors in vivo within a…Continue Reading In vivo quantification of tumor receptor binding potential with dual-reporter molecular imaging

Protoporphyrin IX fluorescence contrast in invasive glioblastomas is linearly correlated with Gd enhanced magnetic resonance image contrast but has higher diagnostic accuracy

J Biomed Opt. 2011 Sep;16(9):096008. doi: 10.1117/1.3622754. ABSTRACT The sensitivity and specificity of in vivo magnetic resonance (MR) imaging is compared with production of protoporphyrin IX (PpIX), determined ex vivo, in a diffusely infiltrating glioma. A human glioma transfected with green fluorescent protein, displaying diffuse, infiltrative growth, was implanted intracranially in athymic nude mice. Image…Continue Reading Protoporphyrin IX fluorescence contrast in invasive glioblastomas is linearly correlated with Gd enhanced magnetic resonance image contrast but has higher diagnostic accuracy

High vascular delivery of EGF, but low receptor binding rate is observed in AsPC-1 tumors as compared to normal pancreas

Mol Imaging Biol. 2012 Aug;14(4):472-9. doi: 10.1007/s11307-011-0503-5. ABSTRACT PURPOSE: Cellular receptor targeted imaging agents present the potential to target extracellular molecular expression in cancerous lesions; however, the image contrast in vivo does not reflect the magnitude of overexpression expected from in vitro data. Here, the in vivo delivery and binding kinetics of epidermal growth factor…Continue Reading High vascular delivery of EGF, but low receptor binding rate is observed in AsPC-1 tumors as compared to normal pancreas

Comparing implementations of magnetic-resonance-guided fluorescence molecular tomography for diagnostic classification of brain tumors

J Biomed Opt. 2010 Sep-Oct;15(5):051602. doi: 10.1117/1.3483902. ABSTRACT Fluorescence molecular tomography (FMT) systems coupled to conventional imaging modalities such as magnetic resonance imaging (MRI) and computed tomography provide unique opportunities to combine data sets and improve image quality and content. Yet, the ideal approach to combine these complementary data is still not obvious. This preclinical…Continue Reading Comparing implementations of magnetic-resonance-guided fluorescence molecular tomography for diagnostic classification of brain tumors

Imaging targeted-agent binding in vivo with two probes

J Biomed Opt. 2010 May-Jun;15(3):030513. doi: 10.1117/1.3449109. ABSTRACT An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3…Continue Reading Imaging targeted-agent binding in vivo with two probes

Fluorescent molecular imaging and dosimetry tools in photodynamic therapy

Methods Mol Biol. 2010;635:207-22. doi: 10.1007/978-1-60761-697-9_15. ABSTRACT Measurement of fluorescence and phosphorescence in vivo is readily used to quantify the concentration of specific species that are relevant to photodynamic therapy. However, the tools to make the data quantitatively accurate vary considerably between different applications. Sampling of the signal can be done with point samples, such…Continue Reading Fluorescent molecular imaging and dosimetry tools in photodynamic therapy

MRI-coupled fluorescence tomography quantifies EGFR activity in brain tumors

Acad Radiol. 2010 Mar;17(3):271-6. doi: 10.1016/j.acra.2009.11.001. ABSTRACT RATIONALE AND OBJECTIVES: This report demonstrates the diagnostic potential of magnetic resonance imaging (MRI)-coupled fluorescence molecular tomography (FMT) to determine epidermal growth factor receptor (EGFR) status in brain cancer. MATERIALS AND METHODS: Two orthotopic glioma xenograft models were used in this study: one represented high EGFR expression and…Continue Reading MRI-coupled fluorescence tomography quantifies EGFR activity in brain tumors

Imaging tumor variation in response to photodynamic therapy in pancreatic cancer xenograft models

Int J Radiat Oncol Biol Phys. 2010 Jan 1;76(1):251-9. doi: 10.1016/j.ijrobp.2009.08.041. ABSTRACT PURPOSE: A treatment monitoring study investigated the differential effects of orthotopic pancreatic cancer models in response to interstitial photodynamic therapy (PDT), and the validity of using magnetic resonance imaging as a surrogate measure of response was assessed. METHODS AND MATERIALS: Different orthotopic pancreatic…Continue Reading Imaging tumor variation in response to photodynamic therapy in pancreatic cancer xenograft models

Detecting epidermal growth factor receptor tumor activity in vivo during cetuximab therapy of murine gliomas

Acad Radiol. 2010 Jan;17(1):7-17. doi: 10.1016/j.acra.2009.07.027. Epub 2009 Sep 30. ABSTRACT RATIONALE AND OBJECTIVES: Noninvasive molecular imaging of glioma tumor receptor activity was assessed with diagnostic in vivo fluorescence monitoring during targeted therapy. The study goals were to assess the range of use for treatment monitoring and stratification of tumor types using epidermal growth factor…Continue Reading Detecting epidermal growth factor receptor tumor activity in vivo during cetuximab therapy of murine gliomas