Location:
Vail Building, Room 309
Geisel School of Medicine, Hanover, NH
603.646.5182
INTERACTIONS AND IMAGING
The Molecular Interactions and Imaging Core (MIIC) enables research projects by offering support on numerous methodologies to characterize biomolecular interactions. In particular, we provide expertise on sample preparation (working with the MTC), data collection, and analysis for a range of instrumentation including surface plasmon resonance (label-free analysis of biomolecular interactions) and numerous measurements using absorbance, luminescence, and fluorescence (fluorescence anisotropy, alpha-screen, near-infrared fluorescence). The MIIC can facilitate access to additional instrumentation and various applications to characterize biological systems, including drug target identification and validation, such as isothermal calorimetry and nuclear magnetic resonance.
![](https://sites.dartmouth.edu/biomt/files/2019/10/DSC_0104.jpg)
AVAILABLE EQUIPMENT IN THE MIIC:
(for more information, including additional equipment details, reserving equipment, and requesting training, visit RaDar)
Equipment | Description |
---|---|
Synergy Neo2 Hybrid Multi-Mode Microplate Reader | For numerous HTS applications with multi-detection modes. |
LI-COR Odyssey Clx | For quantitative, highly sensitive near-infrared fluorescence detection. |
DeNovix DS-11 FX+Spectrophotometer/Fluorometer | For a variety of micro-volume nucleic acid and protein analyses. |
Bio-Rad GelDocXR | For high-resolution nucleic acid and protein gel imaging. |
Biacore X100 | Measurement of biomolecular interactions in real-time. |
MICROSCOPY
Contact:
Zdenek Svindrych, Ph.D.
Pat Robison, Ph.D.
We provide microscopy support to the bioMT community. With a broad range of microscopes available at Dartmouth (either located in one of the imaging cores – Class of 1978 Life Sciences Center and Dartmouth-Hitchcock Medical Center, or in individual labs), we can cover many applications. The MIIC uses microscopy for both biochemical and cellular studies and assists investigators with designing and implementing methodologies to answer specific research questions.
![](https://sites.dartmouth.edu/biomt/files/2019/10/DSC_0186.jpg)
AVAILABLE MICROSCOPES AND IMAGE-PROCESSING SOFTWARE:
Type of Microscope | Microscope Name | Description | Location |
---|---|---|---|
Laser Scanning Confocal | Zeiss LSM880 | With Airyscan; live cell incubation capabilities | Imaging Facility, Biology/Life Sciences Center |
Laser Scanning Confocal | Zeiss LSM800 | With Airyscan; live cell incubation capabilities | Microscopy Facility, DHMC, IPIM |
Spinning Disk Confocal | Andor W1 | With Mosaic and Micropoint photostimulation/photoablation | Imaging Facility, Biology/Life Sciences Center |
Spinning Disk Confocal | Nikon Ti-E-inverted | Nikon Ti-E-inverted | Imaging Facility, Biology/Life Sciences Center |
Total Internal Reflection | Nikon/Andor TIRF | Nikon/Andor TIRF | Imaging Facility, Biology/Life Sciences Center |
Total Internal Reflection | Olympus TIRF Olympus IX-83 | Live cell incubation capabilities | Microscopy Facility, DHMC, IPIM |
Multiphoton | Olympus FVMPE-RS | With MaiTai InSight FLIM/FCS | Imaging Facility, Biology/Life Sciences Center |
Epifluorescence | Nikon 90i | Upright | Imaging Facility, Biology/Life Sciences Center |
Epifluorescence | Nikon SMZ1500 | Dissecting stereo zoom | Imaging Facility, Biology/Life Sciences Center |
Superresolution | Zeiss PS.1 PALM/SIM | Zeiss PS.1 PALM/SIM | Imaging Facility, Biology/Life Sciences Center |
Slide Scanner | PerkinElmer Vectra3 | Automated imaging of up to 6 slides | Microscopy Facility, DHMC, IPIM |
Image-Processing Software | Imaris Workstation | With Measurement Pro, ImarisTrack, ImarisColoc, ImarisXT, ImarisCell, ImarisVantage, and Filament Tracker | Imaging Facility, Biology/Life Sciences Center |
Image-Processing Software | NIS Elements Workstation | With Deconvolution | Imaging Facility, Biology/Life Sciences Center |
Image-Processing Software | Arivis Vision4d | Powerful visualization and analysis of multidimensional images | Imaging Facility, Biology/Life Sciences Center |
Image-Processing Software | Analysis Workstation | Includes image analysis software packages HALO3 and InForm | Microscopy Facility, DHMC, IPIM |