bioMT provides two scientific cores which provide state-of-the art equipment, experienced staff, and training to support local and regional researchers. Detailed information can be found on each of Core’s websites.
Download a copy of our Scientific Cores brochure!
Molecular Tools Core and Molecular Interactions & Imaging Core brochure (pdf)
Molecular Tools Core (MTC)
The Molecular Tools Core is a bioMT and Dartmouth community resource that enables research projects via acquisition or production of high quality recombinant proteins. We are developing optimization strategies spanning the molecular, cellular, and process levels in order to build an integrated pipeline capable of producing a wide spectrum of proteins and protein domain fragments.
Director:
Dale Mierke, Ph.D.
Contact:
Andreia Verissimo, Ph.D.
Susu He, PhD
Angela Kull, M.S.
bioMT Service Center – NEW!
The bioMT Service Center is our new model for delivery of cell culture services to the Dartmouth community. We currently offer recombinant protein production in human (Freestyle 293-F and Expi293F) and insect (Sf9 and High Five) cell lines using transient transfection. Cell culture and transfection services are subsidized by our COBRE program (NIH IDeA award for bioMT P20-GM113132). This service, executed by experienced cell culture staff, is available to Dartmouth users on a first-come-first-served basis. We comply with good cell culture practices, including routine cell passage, viability monitoring, Mycoplasma testing ( as needed) and sterility. Transfection and protein expression are done according to well established protocols for high-yield protein production.
For questions contact Andreia Verissimo or Emilie Shipman or visit our bioMT Service Center information in RaDar for more information.
Molecular Interactions & Imaging Core
To quantitatively characterize the molecular interaction of biomolecular systems, the MIIC will provide expertise in the sample preparation (working with the MTC), data collection, and analysis for a range of instrumentation including isothermal calorimetry, surface plasmon resonance, fluorescence-based measurements (fluorescence polarization, alpha-screen), and nuclear magnetic resonance.
Director:
Henry Higgs, Ph.D.
Contact:
Zdenek Svindrych, Ph.D.
Pat Robison, Ph.D.
Andreia Verissimo, Ph.D.,
Angela Kull, M.S.
Microscopy
We provide microscopy support to the bioMT community. With a broad range of microscopes available at Dartmouth (either located in one of the imaging cores – Class of 1978 Life Sciences Center and Dartmouth-Hitchcock Medical Center, or in individual labs), we can cover many applications. The MIIC uses microscopy for both biochemical and cellular studies, and will help individuals design and implement methodologies to answer specific research questions.
Contact:
Zdenek Svindrych, Ph.D.
Pat Robison, Ph.D.