The Center for Quantitative Biology’s Single Cell Genomics Core has recently enabled Spatial Transcriptomics capabilities by implementing the 10x Genomics Visium workflow. As part of our effort to gain experience with new tissue/sample types and to promote the method across campus, we are offering 1:1 matching funds for a limited number of 10x Visium experiments. Each project will include a tissue optimization step specific to the chosen sample, and a full Gene Expression Slide capable of interrogating 4 tissue sections, 6.6mm^2 or less in size. Additional criteria include:
- Samples must be snap frozen in OCT, ideally following the 10x protocol. FFPE samples cannot be processed at this time.
- Samples must pass RNA quality check (performed in the core).
All steps of the workflow, including tissue staining, imaging, library preparation and sequencing will be performed in the Core. Cryosectioning of tissue blocks is performed in close coordination with the Pathology Shared Resource.
If interested, please email a ½ page project summary/scope, as a PDF, to Fred Kolling (fwk@dartmouth.edu), no later than February 12, 2021. Projects with competition dates prior to April 1, 2021 will be prioritized to accommodate the short shelf life of Visium reagents.
If you would like to discuss the details of your project, please email Fred Kolling (fwk@dartmouth.edu).