Co-Directors: Bruce Stanton, PhD, Andrew C. Vail Professor of Microbiology and Immunology and Deborah Hogan, PhD, Professor of Microbiology and Immunology
Core support staff: Roxanna Barnaby and Amanda Nymon
Location: Remsen Building (rooms 504 and 519) on the Hanover campus of the Geisel School of Medicine.
Description:
The GIBC offers a broad spectrum of model systems, expertise in the selection of models and analytical approaches, and the introduction or development of new model systems to facilitate GI research by DartCF scientists. The GIBC has implemented a suite of models to study GI disease in CF, including paired WT and CF mouse and human 3D intestinal organoids, 2D monolayers derived from intestinal organoids, Gut-On-A-Chip systems, engineered cell lines, transformation reagents and mouse models. The human GI cell models have been developed in collaboration with the Clinical Research Translation Core (CRTC), which have provided biopsy tissue samples and GI clinical bacterial isolates. GIBC services include support for the following:
- GI host-microbe co-culture studies. The GIBC has implemented 3D organoids and 2D organoid-derived monolayers from mice and humans, leveraged its expertise in co-culture models to study interactions between these models and GI bacteria, initiated the Gut-On-A-Chip model, and characterized over 30 bacterial isolates obtained from the stool of CF and non-CF children and from endoscopic aspirates (CTRC)
- Imaging studies of gut epithelial cells and bacterial biofilms. The core has a Keyence BZ800 microscope and access to several confocal microscopes at Dartmouth.
- Expertise in single cell-RNA-sequencing, other molecular techniques, and characterization of GI organoids.
- Functional studies including forskolin-induced swelling assays (FIS) of 3D GI organoids, and electrophysiological studies of 2D organoid monolayers.
- Studies on mouse models to study GI disease in CF.
- Access to GI bacterial and fungal strains.
- Coordination with industry to develop new multi-organ therapeutics relevant to CF.
- Training in the use of GI microbe-host co-culture organoid models and mouse models.
Major Equipment in the P30 GIBC:
| Equipment | Quantity |
|---|---|
| Easy Mount Diffusion Chamber and Clamp for Ussing studies | 6 |
| Beckman Coulter Z1 Particle Counter | 1 |
| BioRad TC10-automatic cell counter | 1 |
| Nanodrop ND-1000 Spectrophotometer | 1 |
| Agilent 2100 Bioanalyzer | 1 |
| NanoSight NS300 | 1 |
| A hood for virus and mouse work | 1 |
| Emulate Zoe and the Orb (Lung on a Chip) | 1 |
| Keyence BZ-X800 fluorescence microscope for biofilm studies | 1 |
Keyence BZ-X800 epi fluorescence microscope (filter cube sets):
| DAPI, GFP, TRITC and Texas Red |
| DAPI: Excite 360/40, emission: 460/50 |
| DAPI-V for sectioning: ex: 395/25 em:460/50 |
| GFP: ex 470/40, em 525/50 |
| TRITC: ex 545/25, em 605/70 |
| Texas red: ex 560/40, em 630/75 |